Saturday, February 14, 2015

How to Extract DNA from a Strawberry

How does it work?
Strawberries have more DNA than any other fruit with 8 pairs in each chromosome. To extract the DNA, each of the solutions used in the experiment plays a part. The soap dissolves the cell membrane, which frees the chromosomes from the nuclear membrane. The salt is added to break up the nucleotides in the DNA sequence. Finally, DNA is not soluble in alcohol because it is polar, and even less so when the alcohol is chilled.

Materials-
A strawberry
A plastic bag
10 ml of soapy salt water
100% alcohol solution
Pipett
A coffee filter
A clear test tube

Procedure
1) Rip off the green part of the strawberry
2) Put strawberry in plastic bag and mush up until pulped
3) Add 10 ml of the soapy solution to the bag, seal, and then continue to squish some more. 
4) Put cone into test tube, and put coffee filter into the cone
5) Slowly pour strawberry gunk into coffee filter
6) Allow the strawberry juice to filter through the coffee filter, it will take a few minutes
7) Add 2 ml of cold 100% alcohol to the strawberry juice
8) Watch for a minute and the line between the red juice and the clear solution will become foggy.
9) Put stirrir into foggy area and twirl
10) Pull out the stirrer, see that snot looking stuff? That's DNA!